| Solution Information | help | |
| Enzyme: | Ephrin type-B receptor 4 | |
| inhibitor: | BDBM41476 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose response curves for 128 available out of 143 compounds identified as active in a previously described set of experiments entitled "Primary biochemical high-throughput screening assay for antagonists of the interaction between the Eph receptor B4 (EphB4) and its ligand ephrin-B2 via TNYL-RAW peptide" (PubChem AID = 720). As with the primary assay, this assay is based on the ability of the ligand binding domain of EphB4 (LBD-EphB4) protein to form a complex with the ephrin-B2-antagonistic peptide, TNYL-RAW. TNYL-RAW has been labeled with Alexa-532 dye in order to monitor its interaction with EphB4 by fluorescence polarization. High fluorescence polarization is evidence of receptor-ligand interaction. Low fluorescence polarization reflects the disruption or absence of receptor-ligand interaction. Protocol Summary: To begin the assay 2.5 ul of assay buffer (0.1% Pluronic acid, 50 mM Tris HCl, 150 mM NaCl, 1.0 mM Ca | |
| If you find an error in this entry please send us an E-mail | ||